实验步骤
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DC cell subsets from mouse
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Frequency of Different DC Subset in lymphoid tissue
Cell subset Frequency Markers Function Plasmacytoid DCs
(pDCs)0.5% in spleen;
0.2% in lymph nodes
CD45, CD11c, MHC class II,
mPDCA-1 (CD317 or BST2),
Siglec-H, CD45R (B220), Ly-6C
Upon pathogen encounter,
pDCs produce large
amounts of type I IFN
and acquire antigen-presenting
capacity.
Conventional DC 1
(cDC1)0.5% in spleen;
0.1% in lymph nodes
CD45, CD11c, MHC class II,
CD8a, XCR1, CD24, CLEC9A
cDC1 perform cross-presentation
of antigens to MHC class I
and start type I cytotoxic
immune responses.
Conventional DC 2
(cDC2)1% in spleen;
0.2% in lymph nodes
CD45, CD11c,
MHC class II,
CD4, SIRPα, CD11b
cDC2 activate innate lymphoid
cells 2 (ILC2) and TH2 cells
and Induce ILC3 and
TH17 immune responses.
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Frequency of Different DC Subset in non- lymphoid tissue
Cell subset
Frequency
(percentages refer
to total CD45+ cells)
Markers Function Plasmacytoid DCs (pDCs) Rare at steady state,
variable during inflammation
CD45, CD11c, MHC class II,
mPDCA-1 (CD317 or BST2),
Siglec-H, CD45R (B220), Ly-6C
Upon pathogen encounter
pDCs produce large amounts
of type I IFN and acquire
antigen-presenting capacity
Conventional DC 1 (cDC1) 1% in skin,
0.5% in lungs,
1% in intestines
CD45, CD11c, MHC class II,
CD103, XCR1, CD24, CLEC9A
Perform cross-presentation
of antigens to MHC class
I and start type I
cytotoxic immune responses
Conventional DC 2 (cDC2) 25% in skin,
1.5% in lungs,
0.45% in intestines
CD45, CD11c,
MHC class II, SIRPα, CD11b
Activate ILC2 and TH2 cells
and induce ILC3 and
TH17 immune responses
Inflammatory DCs Variable,
depending on inflammation
CD11c, MHC class
II MoDCs: CD64, F4/80,
MER (MERTK), CD11b
Induce activation
of naive T cells
Langerhans cells 30–50% in skin Located in skin to
take up microbial antigens
and become APCs