RBPSUH (D10A4) Rabbit mAb

RBPSUH (Recombining Binding Protein, SUppressor of Hairless), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (1-3). RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5). 1.Ehebauer, M. et al. (2006) Sci STKE 2006, cm7. 2.Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46. 3.Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33. 4.Henkel, T. et al. (1994) Science 265, 92-5. 5.Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.