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SULT1E1
SULT1E1
品牌： Usbiological
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对比
咨询纯度：
Purified by immunoaffinity chromatography.
反应种属：
Rabbit
产品介绍
产品介绍
产品信息
抗原来源
Human
宿主
Rabbit
免疫原
Synthetic peptide corresponding to human SULT1E1. Species sequence homology: Porcine (89%), Equine (100%), Rabbit (89%)
产品用途
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.
简单描述
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
同种型
IgG
克隆类别
Polyclonal
纯度
Purified by immunoaffinity chromatography.
形式
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
应用
实验应用
E IC IF IHC WB
推荐用量
Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
反应种属
Rabbit
交叉反应性
Hu Mo Rt
目标/特异性
Recognizes endogenous levels of human SULT1E1. |Species Crossreactivity: mouse and rat
稀释度
Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight. Immunohistochemistry: 1:50-1:200 Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
背景
别名
EST, EST-1, EST1, Estrogen sulfotransferase, estrogen-preferring, estrone sulfotransferase, ST1E1, ST1E1_HUMAN, STE, Sulfotransferase 1E1, Sulfotransferase, Sulfotransferase estrogen preferring, SULT1E1
制备和贮存
保存方式
Storage_Temp:-20°C
Storage_and_Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
数据库链接
UniProt ID
P49888
SwissProt
P49888
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