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BD Pharmingen™ Dog Activated T Lymphocyte Cocktail

品牌： BD Pharmingen

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产品介绍

产品信息

简单描述

The Dog Lymphocyte Activation Cocktail is a three-color reagent cocktail designed to identify dog activated lymphocytes by direct immunofluorescent staining with flow cytometric analysis. The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes. The LSM 11.425 antibody was generated against cells derived from canine peripheral lymph nodes and has been used as a prognostic tool in dog B cell lymphoma studies. The CTL 2.58 antibody was generated by using whole cell immunizations of IL-2 dependent feline T cell lines stimulated with PHA and Con A. This clone reacts with dog and is referred to as the T Cell Activation Marker.

克隆号

(RUO)

BD化合物表

描述
数量/尺寸
零件号
EntrezGene ID
APC Mouse anti-Dog Pan T Cell
N/A
51-590-9005060
N/A
Multiple Mouse anti-Dog B Cells
N/A
51-550-9003756
N/A
FITC Mouse anti-Dog Activated T cells
N/A
51-540-9005068
N/A

应用

反应种属

Dog (QC Testing)

目标/特异性

immunotox cocktail kit

文献

研发参考(3) 1. Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994; 43:137-154. (Clone-specific). 2. Gebhard GH, Carter PB. Identification of canine T-lymphocyte subsets with monoclonal antibodies. Vet Immunol Immunopathol. 1992; 33:187-199. (Biology). 3. Ruslander DA, Gebhard DH, Tompkins MB, Grindem CB, Page RL. Immunophenotypic characterization of canine lymphoproliferative disorders. In Vivo. 1997; 11(2):169-172. (Biology).

参考图片

Three-color analysis of the expression of LSM 8.358 (CD3/Pan T marker), LSM 11.425 (B cell marker) and CTL 2.58 (activation marker) on lysed whole blood from Canine . PBMC from Dog was stained with either Isotype Control Cocktail A (Cat. no. 558509; data not shown) or Dog Activated T Lymphocyte Cocktail (Cat. no. 558704). During data analysis, lymphocytes were identified by scatter profile. The figure on the left represents the LSM 8.358 (Pan T marker) and CTL 2.58 (activation marker) profile on resting lymphocytes while the figure in the middle represents these markers on Concanavalin A stimulated PBMC. The panel on the right represents the profile for LSM 8.58 (Pan T marker) and LSM 11.425 (B cell marker) also on Concanavalin A stimulated cells. Flow cytometry was performed on a BD FACSCalibur™.

Three-color analysis of the expression of LSM 8.358 (CD3/Pan T marker), LSM 11.425 (B cell marker) and CTL 2.58 (activation marker) on lysed whole blood from Canine. Dog PBMC were stained with Dog Activated T Lymphocyte Cocktail (Cat. No. 558704). During data analysis, lymphocytes were identified by scatter profile. The figure on the left represents the LSM 8.358 (Pan T marker) and CTL 2.58 (activation marker) profile on resting lymphocytes while the figure in the middle represents these markers on Concanavalin A-stimulated PBMC. The panel on the right represents the profile for LSM 8.58 (Pan T marker) and LSM 11.425 (B cell marker) on Concanavalin A-stimulated cells. Flow cytometry was performed on a BD FACSCalibur™.

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货号：

558704

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