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69kDa (Reducing)
10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol pH 7.4 @ 25°C
Name | Storage Temperature | quantity | concentration |
T4 RNA Ligase 1 | -20 ℃ | 100 ul or 500 µl | 10 U/µl |
10*Reaction Buffer | -20 ℃ | 1 ml | 10 X |
Adenosine 5'-Triphosphate (ATP) | -20 ℃ | 1 ml | 10 mM |
PEG8000 (RNase free) | -20 ℃ | 1 ml | 50% |
2μg (R: reducing condition, N: non-reducing condition).
"}]T4 RNA Ligase 1 is an ATP-dependent enzyme that catalyzes the formation of phosphodiester bonds between the 5'-P and 3'-OH ends of single-stranded RNA, single-stranded DNA, or single nucleotides, either intermolecular or intramolecular. It is suitable for the connection of single-stranded RNA molecules and the 5' end junction constructed by miRNA libraries in NGS. Labeled with RNA 3 'terminal; Cyclization of oligonucleotides; tRNA modification; In 5'-RACE (Rapid Amplification of cDNA Ends) assay, it is used for oligonucleotides to bind to single-stranded cDNA; and the introduction of unnatural amino acids into proteins.
Store at -25 ~ -15℃ for 2 years
[1] England, Thomas E , and O. C. Uhlenbeck . "3|[prime]|-Terminal labelling of RNA with T4 RNA ligase." Nature 275.5680(1978):560-1.
[2] Tessier, Daniel C. , R. Brousseau , and T. Vernet . "Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase." Analytical Biochemistry 158.1(1986):171-178.