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对比
咨询Component | UA070079-50 Rxns | UA070079-100 Rxns |
T7 RNA Polymerase (50 U/μL) | 50 μl | 100 μl |
RNase Inhibitor (40 U/μL) | 25 μl | 50 μl |
DNase I, RNase-free (2 U/μL) | 100 μl | 200 μl |
10×Transcription Buffer | 100 μl | 200 μl |
ATP (100 mM) | 100 μl | 200 μl |
UTP (100 mM) | 100 μl | 200 μl |
GTP (100 mM) | 100 μl | 200 μl |
CTP (100 mM) | 100 μl | 200 μl |
Control Template (0.5 μg/μl) | 10 μl | 20 μl |
RNase Free dH2O | 1 ml | 1 ml×2 |
Store at -25 ~ -15℃ for 2 years
[1] Athanasios D , Kanchana R ,Hobert Elissa M.Moore Melissa J.Rabideau Amy E.An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts[J].Nature biotechnology, 2023, 41(4):560-568.
[2]Skinner, and M. G. . "Promoter Binding, Initiation, and Elongation By Bacteriophage T7 RNA Polymerase A SINGLE-MOLECULE VIEW OF THE TRANSCRIPTION CYCLE." Journal of Biological Chemistry 279.5(2004):3239-44.
参考图片
By using T7 High Yield Transcription Kit of self-developed and competing products, PCR products with T7 Promoter were used as templates for vitro transcription. In the 20µl reaction system, PCR products containing T7 Promoter were added as template DNA, and the reaction was incubated at 37℃ for 2h, and incubated at 70℃ for 10min to terminate the reaction. 2U DNase I was added to digest the template DNA, and the RNA transcription product obtained was 133nt. 5μl reaction product was removed, 1μl 6X DNA Loading Buffer was added, denatured at 95℃ for 5min, and then 1% agarose gel was used for electrophoresis at room temperature, 1X TAE was used as the electrophoresis solution, and 130V was used for 20min. After electrophoresis, the results were photographed. As shown in the figure, this product has a consistent transcription effect compared with competing products.
Lane 1 Negative Control (Only negative control without enzyme added);
Lane 2 T7 High Yield RNA Synthesis Kit (UA);
Lane 3 T7 High Yield RNA Synthesis Kit (Competitor N)