




Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TACI/TNFRSF13B/CD267 protein.


Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Flow Cytometry (Live) | 1:50 - 1:200 |





Specificity/Sensitivity
Species Reactivity:
Human




Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


参考图片
Flow cytometric analysis of fixed and permeabilized LoVo cells (blue, negative) and REC-1 cells (green, positive) using TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from REC-1, L-363, and Raji cells using TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of TACI/TNFRSF13B/CD267 protein from REC-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb. Western blot analysis was performed using TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.
Confocal immunofluorescent analysis of REC-1 cells (left, positive) or LoVo cells (right, negative) using TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb (green). Nuclei were labeled with DAPI #4083 (blue).
Flow cytometric analysis of live human peripheral blood mononuclear cells co-stained with CD19 (SJ25C1) Mouse mAb (APC Conjugate) using TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb (right) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow cytometric analysis of live LoVo cells (blue, negative) and REC-1 cells (green, positive) using TACI/TNFRSF13B/CD267 (E1D1P) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.