产品介绍
产品信息
简单描述
The FastScan™ Total Tau ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tau. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with tau in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of tau.
*Antibodies in this kit are custom formulations specific to kit.
组成成分
微管相关蛋白兔单克隆抗体0.16%，微管相关蛋白兔单克隆抗体、偶联HRP（辣根过氧化物酶）0.16%，微管相关重组蛋白0.16%，检测抗体稀释液（磷酸盐缓冲液）4.67%,辣根过氧化物酶稀释液（三(羟甲基)氨基甲烷溶液)4.67%，TMB底物（三(羟甲基)氨基甲烷溶液)17.11%，终止液（顺丁烯二酸溶液）17.11%，洗涤缓冲液（0.5%5-氯-2-甲基-3(2H)异噻唑酮、2-甲基3(2H)异噻唑酮溶液）38.88%，细胞提取缓冲液 (聚乙二醇单辛基苯基醚水溶液)15.55%，细胞提取增强剂溶液 (十二烷基硫酸钠)1.53%
应用
目标/特异性
Specificity/Sensitivity
The FastScan™ Total Tau ELISA Kit detects endogenous levels of tau as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human, Mouse, Rat
背景
背景
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
1.Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2.Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3.Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
参考图片
Figure 1. Tau protein is expressed in mouse brain but absent in HeLa cells. The relationship between lysate protein concentration from mouse brain and HeLa cells and the absorbance at 450 nm using the FastScan™ Total Tau ELISA Kit #57519 is shown in the upper figure. The corresponding western blot using a Tau antibody is shown in the lower figure. Mouse brain tissue or unstarved HeLa (90% confluence) cells were collected and then lysed.
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