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产品介绍
产品介绍
产品信息
荧光素标记
宿主
rabbit
免疫原
Synthetic peptide corresponding to AA 1 to 6 from TEV-cut site
产品用途
Excess of extracellular membrane bound SynaptoPhluorin is often removed by TEV protease digestion. This antibody recognizes the neoepitopes generated by TEV cleavage and can be used for membrane internalization studies. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.
简单描述
50 µg specific antibody, lyophilized
应用
实验应用
ICC
目标/特异性
TEV-cut protease site is recognized with very strong preference.
背景
背景
TEV is a highly specific cysteine protease derived from Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence (ENLYFQ(G/S)) and cleavage occurs between the Q and G/S residues leaving a free carboxy-terminus.1 ) Sun C, Protein expression and purification (2012) 821: 226-31. 2 ) Lundbäck AK, Analytical biochemistry (2008) 3821: 69-71. 3 ) Phan J, The Journal of biological chemistry (2002) 27752: 50564-72. 4 ) Kapust RB, Biochemical and biophysical research communications (2002) 2945: 949-55. 5 ) Kapust RB, Protein engineering (2001) 1412: 993-1000.
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