



Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro413 of mouse TFEB protein.


Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |



Specificity/Sensitivity
Species Reactivity:
Mouse




Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


参考图片
Immunoprecipitation of TFEB from A20 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TFEB (D4L2P) Rabbit mAb. Western blot was performed using TFEB (D4L2P) Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Western blot analysis of extracts from A20, CTLL-2, and EL4 cell lines using TFEB (D4L2P) Rabbit mAb.
Western blot analysis of extracts from 634T wild-type or two 634T cell lines with a TFEB knockout using TFEB (D4L2P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Knockout cells were kindly provided by Dr. Reuben Shaw, Salk Institute for Biological Studies, La Jolla, CA.