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TFEB (D4L2P) Rabbit mAb
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TFEB (D4L2P) Rabbit mAb

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品牌: CST
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分子量:
65-70
来源宿主:
Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro413 of mouse TFEB protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunoprecipitation1:200
同种型
Rabbit IgG
分子量
65-70
应用
目标/特异性

Specificity/Sensitivity

TFEB (D4L2P) Rabbit mAb recognizes endogenous levels of total mouse TFEB protein.

Species Reactivity:

Mouse

敏感性
Endogenous
背景
背景
Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the upregulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in upregulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3). 1.Sardiello, M. et al. (2009) Science 325, 473-7. 2.Sardiello, M. and Ballabio, A. (2009) Cell Cycle 8, 4021-2. 3.Settembre, C. et al. (2011) Science 332, 1429-33. 4.David, R. (2011) Nat Rev Mol Cell Biol 12, 404. 5.Cuervo, A.M. (2011) Science 332, 1392-3. 6.Peña-Llopis, S. et al. (2011) EMBO J 30, 3242-58. 7.Settembre, C. and Ballabio, A. (2011) Autophagy 7, 1379-81. 8.Peña-Llopis, S. and Brugarolas, J. (2011) Cell Cycle 10, 3987-8. 9.Settembre, C. et al. (2012) EMBO J 31, 1095-108. 10.Martina, J.A. et al. (2012) Autophagy 8, 903-14.
研究领域
癌症,细胞生物学,纤维化,代谢,神经科学
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接
Entrez-Gene ID
21425
UniProt ID
Q9R210

参考图片

Immunoprecipitation of TFEB from A20 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TFEB (D4L2P) Rabbit mAb. Western blot was performed using TFEB (D4L2P) Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

Western blot analysis of extracts from A20, CTLL-2, and EL4 cell lines using TFEB (D4L2P) Rabbit mAb.

Western blot analysis of extracts from 634T wild-type or two 634T cell lines with a TFEB knockout using TFEB (D4L2P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Knockout cells were kindly provided by Dr. Reuben Shaw, Salk Institute for Biological Studies, La Jolla, CA.

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货号:
32361T
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20μl
100ul
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