全部商品分类首页
THUNDER™ cAMP TR-FRET Assay Kit
用小程序，查商品更便捷
产品介绍
产品介绍
产品信息
抗原名称
cAMP
宿主
鼠
商品描述
Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. This is a robust, sensitive, and easy-to-use assay for monitoring GPCR activation based on cAMP production in cells. The kit is compatible with both suspension and adherent cells and is suitable for the profiling and high-throughput screening of Gαs- and Gαi-coupled GPCRs. Detailed assay protocols for testing agonists and antagonists, as well as full assay validation can be found in the Technical Data Sheet. BEST SELLER
组成成分
腺苷3',5'-环单磷酸8.3%，生物素化腺苷3',5'-环单磷酸2.08%，链霉亲和素2.08%，抗腺苷3',5'-环单磷酸抗体4.2%，检测缓冲液（磷酸盐缓冲液）83.34%
Components
cAMP standard (100 µL)
Biotin-cAMP (25 µL)
Eu-streptavidin (25 µL)
Acc-anti-cAMP (50 µL)
5X cAMP Detection Buffer (2 x 1 mL)
Reactivity
All species expected
Assay principle: Free cAMP produced by cells competes with the Europium labeled streptavidin/biotin-cAMP tracer complex for binding to a monoclonal cAMP antibody labeled with the far-red fluorophore. The specific TR-FRET signal is therefore inversely proportional to the concentration of cAMP in the lysate.
The THUNDER™ cAMP assay exhibits a comparable performance to that of a competing TR-FRET assay.
The THUNDER™ cAMP assay workflow consists of only two steps.
应用
反应种属
All species expected
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。