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THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
ERK1/2
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of low levels of Phospho-ERK1/2 (T202/Y204) in cell lysates. This high-sensitivity kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER
组成成分
极端磷酸化丝裂原活化蛋白激酶抗体（铕离子标记），极端磷酸化丝裂原活化蛋白激酶抗体（远红光受体标记），裂解缓冲液（聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液（三(羟甲基)氨基甲烷溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）
Components
Eu-phospho-XTMERK1/2 (500 µL)
Acc-phospho-XTMERK1/2 (2 mL)
ERK1/2 control lysate (2 x 200 µL)
5X Lysis Buffer 1 (100 mL)
10X TR-FRET Detection buffer (5 mL)
100X Phosphatase Inhibitor Cocktail (5 mL)
Reactivity
Human, Mouse
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.
Data show that treatment of H358 cells with RMC-4550 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
Data show that treatment of MCF7 cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
Data show that treatment of B lymphocyte cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
应用
反应种属
Human, Mouse
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