THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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参数

反应种属：

Human, Mouse

来源宿主：

鼠

产品介绍

产品信息

抗原名称

ERK1/2

宿主

鼠

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of low levels of Phospho-ERK1/2 (T202/Y204) in cell lysates. This high-sensitivity kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

组成成分

极端磷酸化丝裂原活化蛋白激酶抗体（铕离子标记），极端磷酸化丝裂原活化蛋白激酶抗体（远红光受体标记），裂解缓冲液（聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液（三(羟甲基)氨基甲烷溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）

Components
Eu-phospho-XTMERK1/2 (25 µL)
Acc-phospho-XTMERK1/2 (100 µL)
ERK1/2 control lysate (200 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human, Mouse

STIMULATION OF PHOSPHO-ERK1/2 (T202/Y204) IN HEK293 CELLS

Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.

INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN H358 CELLS

Data show that treatment of H358 cells with RMC-4550 inhibits basal phosphorylation of ERK1/2 at T202/Y204.

INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN MCF7 CELLS

Data show that treatment of MCF7 cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.

INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN B LYMPHOCYTES

Data show that treatment of B lymphocyte cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.

应用

反应种属

Human, Mouse

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货号：

KIT-XTM-ERKP-500

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