THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

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品牌: Bioauxilium
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    反应种属:
    Human, Mouse
    Human, Mouse
    产品介绍
    产品信息
    抗原名称
    ERK1/2
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of low levels of Phospho-ERK1/2 (T202/Y204) in cell lysates. This high-sensitivity kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

    Components
    Eu-phospho-XTMERK1/2 (250 µL)
    Acc-phospho-XTMERK1/2 (1 mL)
    ERK1/2 control lysate (2 x 200 µL)
    5X Lysis Buffer 1 (50 mL)
    10X TR-FRET Detection buffer (2.5 mL)
    100X Phosphatase Inhibitor Cocktail (2.5 mL)

    Reactivity
    Human, Mouse

    STIMULATION OF PHOSPHO-ERK1/2 (T202/Y204) IN HEK293 CELLS
    Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.
    INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN H358 CELLS
    Data show that treatment of H358 cells with RMC-4550 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
    INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN MCF7 CELLS
    Data show that treatment of MCF7 cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
    INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN B LYMPHOCYTES
    Data show that treatment of B lymphocyte cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
    应用
    反应种属
    Human, Mouse
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    货号:
    KIT-XTM-ERKP-5000
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