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THUNDER™ High Performance Phospho-STAT1(Y701) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品介绍
产品信息
抗原名称
STAT1
宿主
鼠、大肠杆菌
商品描述
High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human and Mouse Phospho-STAT1 (Y701) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Note: This kit is a new and improved high performance version of catalog number KIT-STAT1P-XXX, which will remain available for purchase.
All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
组成成分
磷酸化信号转导和转录激活因子1抗体（铕离子标记）0.4%，磷酸化信号转导和转录激活因子1抗体（远红光受体标记）1.6%，裂解缓冲液 （聚乙二醇单辛基苯基水溶液）81.8%，检测缓冲液（磷酸盐缓冲液）4%，细胞裂解液（重组信号转导和转录激活因子1蛋白，三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4%
Components
Eu-HP-phospho-STAT1 (25 µL)
Acc-HP-phospho-STAT1 (100 µL)
STAT1 control lysate (200 µL)
5X Lysis Buffer 8 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human, Mouse
Data show the superior S/B ratio obtained with the High Performance Phospho-STAT1 (Y701) Kit compared to the standard version of the kit.
Data show that treatment of HeLa cells with IFNα2b stimulates phosphorylation of STAT1 at Y701. Robust S/B ratio and pharmacology data are obtained after 1h of incubation.
Data show that treatment of HeLa with JAK Inhibitor 1 inhibits phosphorylation of STAT1 at Y701 by IFNα2b. Robust S/B ratio and pharmacology data are obtained after 1h of incubation.
The Z’-factor value of 0.89 indicates that the assay is robust and suitable for HTS.
Data show that the High Performance kit detects mouse Phospho-STAT1 (Y701).
应用
反应种属
Human, Mouse
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