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THUNDER™ Human IL-8 TR-FRET Biomarker Assay Kit
品牌： Bioauxilium
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产品介绍
产品信息
抗原名称
IL-8/CXCL8
商品描述
Sandwich TR-FRET immunoassay for the quantitative measurement of human IL-8 (CXCL8; CGP-1; NAP-1) in cell culture supernatants. The kit does not include a recombinant human IL-8 standard, which must be purchased separately from ProSpec (catalog number CHM-327). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.
Key features and benefits:
- Homogeneous: no wash, easy and rapid protocol
- High sensitivity (15 µL sample), specificity, robustness, and reproducibility
- High-quality antibody pairs ensure lot-to-lot consistency
- Reliable: rigorous development, validation, and quality control testing
- Cost-effective: 5 flexible formats; larger quantities available upon request
Components
Eu-anti-IL-8 (250 µL)
Acc-anti-IL-8 (1 mL)
5X Assay Buffer 1 (10 mL)
Standard curve for the human IL-8 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Standard curve for the human IL-8 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
A head-to-head benchmark comparison of the THUNDER Human IL-8 assay (red curve above) and a competitor TR-FRET Human IL-8 assay (blue curve on the right) was conducted. Standard curves were run using the corresponding reagents and protocols.
The THUNDER assay exhibits a comparable dynamic range and S/B ratio to the Competitor assay. In addition, both assays require the same incubation time (1 hour).
Supernatants from PBMC cells treated for two days with LPS and IL-2 were serially diluted with culture medium and assayed for IL-8. Recovery values between 97-115% of the 4-fold diluted sample demonstrate assay parallelism.
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危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
营业执照(三证合一)