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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit
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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
4EBP1
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note.
Components
Eu-phospho-4EBP1 (25 µL)
Acc-phospho-4EBP1 (100 µL)
4EBP1 control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Treatment of A431 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46.
Treatment of HEK293 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46
Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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