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THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
AKT pan
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT1/2/3 (S473) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER
Components
Eu-phospho-AKTS473 (250 µL)
Acc-phospho-AKTS473 (1 mL)
AKT control lysate (2 x 200 µL)
5X Lysis Buffer 7 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)
Reactivity
Human, Mouse
Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.
Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR FRET ratio values.
Treatment of Jurkat cells with LY294002 inhibits phosphorylation of AKT pan at S473.
Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.
应用
反应种属
Human, Mouse
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