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THUNDER™ Phospho-AKT pan (S473) + Total AKT pan TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
AKT pan
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT 1/2/3 (S473) and Total AKT 1/2/3 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total AKT pan proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
磷酸化蛋白激酶B抗体(铕离子标记）2.4%，磷酸化蛋白激酶B抗体(远红光受体标记)9.6%，总蛋白激酶B抗体(铕离子标记)0.6%，总蛋白激酶B抗体(远红光受体标记)2.4%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）0.6%，检测缓冲液（磷酸盐缓冲液）30%，细胞裂解液（三(羟甲基)氨基甲烷水溶液）24.4%，磷酸酶抑制剂混合物 （N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）30%
400 points for phospho; 100 points for total
Components
Eu-phospho-AKTS473 (20 µL)
Acc-phospho-AKTS473 (80 µL)
Eu-Total AKT (5 µL)
Acc-Total AKT (20 µL)
AKT control lysate (200 µL)
5X Lysis Buffer 7 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human, Mouse
Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.
Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human, Mouse
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