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THUNDER™ Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human
产品介绍
产品介绍
产品信息
抗原名称
BTK
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-BTK (25 µL)
Acc-phospho-BTK (100 µL)
BTK control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of Raji cells with Pervanadate stimulates phosphorylation of BTK at Y223
Data show that treatment of Raji cells with Ibrutinib inhibits phosphorylation of BTK at Y223 by Pervanadate
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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