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THUNDER™ Phospho-c-Met (Y1234/Y1235) TR-FRET Cell Signaling Assay Kit

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产品介绍
产品介绍
产品信息
抗原名称
Met

宿主
鼠

商品描述
Components
Eu-phospho-MET (25 µL)
Acc-phospho-MET (100 µL)
MET control lysate (200 µL)
5X Lysis Buffer 5 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human

Data show that treatment of A431 or HeLa cells with HGF stimulates phosphorylation of c-Met at Y1234/Y1235.

Data show that treatment of HeLa cells with PHA-665752 inhibits phosphorylation of c-Met at Y1234/Y1235 by HGF.

The Z’-factor value of 0.7 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human

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