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THUNDER™ Phospho-c-Met (Y1234/Y1235) TR-FRET Cell Signaling Assay Kit
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THUNDER™ Phospho-c-Met (Y1234/Y1235) TR-FRET Cell Signaling Assay Kit

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品牌: Bioauxilium
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反应种属:
Human
来源宿主:
产品介绍
产品介绍
产品信息
抗原名称
Met
宿主
商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-c-Met (Y1234/Y1235) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 40452841

Components
Eu-phospho-MET (25 µL)
Acc-phospho-MET (100 µL)
MET control lysate (200 µL)
5X Lysis Buffer 5 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human

Stimulation of Phospho-c-Met (Y1234-Y1235) in A431 and Hela cells
Data show that treatment of A431 or HeLa cells with HGF stimulates phosphorylation of c-Met at Y1234/Y1235.
Inhibition of Phospho-c-Met (Y1234-Y1235) in HeLa cells
Data show that treatment of HeLa cells with PHA-665752 inhibits phosphorylation of c-Met at Y1234/Y1235 by HGF.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.7 indicates that the assay is robust and suitable for HTS.
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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货号:
KIT-METP-500
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