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THUNDER™ Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit
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THUNDER™ Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
EGFR
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EGFR (Y1068) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER
Product citation: PubMed 40452841
Components
Eu-phospho-EGFR (25 µL)
Acc-phospho-EGFR (100 µL)
EGFR control lysate (200 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068.
Data show that treatment of A431 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF.
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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