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THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit
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THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human
来源宿主：
鼠、大肠杆菌
产品介绍
产品介绍
产品信息
抗原名称
NF-κB
宿主
鼠、大肠杆菌
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER
Product citation: PubMed 36129967
Components
Eu-phospho-NFkB (5 µL)
Acc-phospho-NFkB (20 µL)
NFkB control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα
The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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