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THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
NF-κB
宿主
鼠、大肠杆菌
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Product citation: PubMed 36129967
组成成分
磷酸化核因子蛋白抗体(铕离子标记)0.4%，磷酸化核因子蛋白抗体(远红光受体标记)1.6%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）81.6%，检测缓冲液（磷酸盐缓冲液）4.1%，细胞裂解液（磷酸化核因子重组蛋白，三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4.1%
Components
Eu-phospho-NFkB (25 µL)
Acc-phospho-NFkB (100 µL)
NFkB control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα
The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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