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THUNDER™ Phospho-NF-kB (S536) + Total NF-kB TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
NF-κB
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) and Total NF-κB in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total NF-κB proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
400 points for phospho; 100 points for total
Components
Eu-phospho-NFkB (20 µL)
Acc-phospho-NFkB (80 µL)
Eu-Total NFkB (5 µL)
Acc-Total NFkB (20 µL)
NFkB control lysate (200 µL)
5X Lysis Buffer 5 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536, but does not affect the levels of total NF-kB
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα, but does not affect the levels of total NF-kB
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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