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THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

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产品介绍
产品介绍
产品信息
抗原名称
p38α MAPK

宿主
鼠

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38α MAPK14 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-p38a (5 µL)
Acc-phospho-p38a (20 µL)
p38a control lysate (100 µL)
5X Lysis Buffer 6 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human

Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38α at T180/Y182

The Z’-factor value of 0.71 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human

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