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THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
p38α MAPK
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38α MAPK14 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
磷酸化P38α丝裂原活化蛋白激酶抗体(铕离子标记）0.4%，磷酸化P38α丝裂原活化蛋白激酶抗体(远红光受体标记)1.6%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）81.6%，检测缓冲液（磷酸盐缓冲液）4.1%，细胞裂解液（三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物 （N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4.1%
Components
Eu-phospho-p38a (25 µL)
Acc-phospho-p38a (100 µL)
p38a control lysate (200 µL)
5X Lysis Buffer 6 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38α at T180/Y182
The Z’-factor value of 0.71 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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