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THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
p38α MAPK
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38α MAPK14 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-p38a (250 µL)
Acc-phospho-p38a (1 mL)
p38a control lysate (2 x 200 µL)
5X Lysis Buffer 6 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)
Reactivity
Human
Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38α at T180/Y182
The Z’-factor value of 0.71 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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