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THUNDER™ Phospho-p38αβγ MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
p38αβγ MAPK
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available.
Components
Eu-phospho-p38 (250 µL)
Acc-phospho-p38 (1 mL)
p38a control lysate (2 x 200 µL)
5X Lysis Buffer 6 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)
Reactivity
Human
Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38αβγ at T180/Y182.
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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