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THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit
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THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
p53
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-p53 (5 µL)
Acc-phospho-p53 (20 µL)
p53 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TRFRET ratio values
应用
反应种属
Human
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