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THUNDER™ Phospho-p70 S6K (T389) TR-FRET Cell Signaling Assay Kit
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THUNDER™ Phospho-p70 S6K (T389) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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咨询反应种属：
Human
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
p70 S6K
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p70 S6K (T389) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-P70S6 (5 µL)
Acc-phospho-P70S6 (20 µL)
P70S6 control lysate (100 µL)
5X Lysis Buffer 3 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human
Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of p70 S6K at T389
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of p70 S6K at T389 by insulin
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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