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THUNDER™ Phospho-RPS6 (S240/S244) TR-FRET Cell Signaling Assay Kit
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产品介绍
产品介绍
产品信息
抗原名称
Ribosomal Protein S6
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S240/S244) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
磷酸化核糖体蛋白抗体(铕离子标记），磷酸化核糖体蛋白抗体(远红光受体标记)，裂解缓冲液（聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液（三(羟甲基)氨基甲烷水溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）
Components
Eu-phospho-RPS6S240 (125 µL)
Acc-phospho-RPS6S240 (500 µL)
RPS6 control lysate (200 µL)
5X Lysis Buffer 4 (25 mL)
10X TR-FRET Detection buffer (1.25 mL)
100X Phosphatase Inhibitor Cocktail (1.25 mL)
Reactivity
Human
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S240/S244
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S240/S244 by insulin
The Z’-factor value of 0.64 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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