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THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human
来源宿主：
鼠、大肠杆菌
产品介绍
产品介绍
产品信息
抗原名称
SMAD2
宿主
鼠、大肠杆菌
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD2 (S465/S467) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Product Citation: PubMed 35664796
组成成分
磷酸化细胞信号转导分子抗体(铕离子标记)0.4%，磷酸化细胞信号转导分子抗体(远红光受体标记)1.6%，裂解缓冲液 （聚乙二醇单辛基苯基水溶液）81.8%，检测缓冲液（磷酸盐缓冲液）4%，细胞裂解液（重组细胞信号转导分子蛋白，三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4%
Components
Eu-phospho-SMAD2 (25 µL)
Acc-phospho-SMAD2 (100 µL)
SMAD2 control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of HeLa cells with TGFβ stimulates phosphorylation of SMAD2 at S465/S467
Data show that treatment of HeLa cells with SB 431542 inhibits phosphorylation of SMAD2 at S465/S467 by TGFβ
The Z’-factor value of 0.65 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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