THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

1/2

THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

来源宿主：

产品介绍

产品信息

抗原名称

STAT3

宿主

鼠

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

BEST SELLER

Note: the Phospho-STAT3 (Y705) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking of THUNDER® Phospho-STAT3 against HTRF® and LANCE® Ultra is shown in the updated Application Note. The THUNDER® assay outperforms the two other TR-FRET assays available (HTRF® and LANCE® Ultra).

Note: a new and improved version of this kit is available: High Performance Phospho-STAT3 (Y705) Assay Kit

Product citation: PubMed 34570089

Components
Eu-phospho-STAT3 (5 µL)
Acc-phospho-STAT3 (20 µL)
STAT3 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

Data show the superior performance of the new Phospho-STAT3 (Y705) Assay compared to the previous version of the assay

Stimulation of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705

Inhibition of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα

The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

The THUNDER assay shows the highest S/B ratios between 1 and 4 hours of incubation

Company A (HTRF) assay shows a comparable S/B ratio to the THUNDER assay only after overnight incubation

Company B (LANCE Ultra) assay shows the lowest S/B ratios at all incubation times

Phospho-STAT3 stimulation in NIH/3T3 cells

Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT3 at Y705.

应用

反应种属

Human, Mouse

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

KIT-STAT3P-100

一键复制

询价

100points

500points

2500points

5000points

10000points

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价