THUNDER™ Phospho-STAT4 (Y693) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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参数

反应种属：

Human

产品介绍

产品信息

抗原名称

STAT4

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT4 (Y693) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 34570089

Components
Eu-phospho-STAT4 (250 µL)
Acc-phospho-STAT4 (1 mL)
STAT4 control lysate (2 x 200 µL)
5X Lysis Buffer 2 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)

Reactivity
Human

Stimulation of phospho-STAT4 (Y693) in B cells

Data show that treatment of B lymphocytes with IFNα2b stimulates phosphorylation of STAT4 at Y693.

Inhibition of phospho-STAT4 (Y693) in B cells

Data show that treatment of B lymphocytes with JAK Inhibitor 1 inhibits phosphorylation of STAT4 at Y693 by IFNα2b.

All-in-one-well phospho-STAT4 (Y693) assay

Data show that stimulation of phospho-STAT4 (Y693) in B lymphocytes can be detected using the 1-plate assay protocol.

Data show a linear relationship between lysate dilutions and TR-FRET ratio value.

应用

反应种属

Human

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货号：

KIT-STAT4P-5000

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询价

5000points

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