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THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human, Mouse
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
STAT5
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT5 (Y694) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER
Product citation: PubMed 37698877; PubMed 34570089
组成成分
磷酸化信号转导和转录激活因子5抗体（铕离子标记），磷酸化信号转导和转录激活因子5抗体（远红光受体标记），裂解缓冲液 （聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液 （三(羟甲基)氨基甲烷溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）
Components
Eu-phospho-STAT5 (25 µL)
Acc-phospho-STAT5 (100 µL)
STAT5 control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human, Mouse
Data show that treatment of A431 cells with EGF stimulates phosphorylation of STAT5 at Y694/Y699.
Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of STAT5 at Y694/Y699 by EGF
The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR FRET ratio values
应用
反应种属
Human, Mouse
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