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THUNDER™ Total AKT pan TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
AKT pan
宿主
鼠、大肠杆菌
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total AKT 1/2/3 (both phosphorylated and non-phosphorylated AKT1/2/3) in cell lysates. This kit can be used to normalize data obtained with the Phospho-AKT pan (S473) kit and the Phospho-AKT pan (T308) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
总蛋白激酶B抗体(铕离子标记)0.4%，总蛋白激酶B抗体(远红光受体标记)1.6%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）81.6%，检测缓冲液（磷酸盐缓冲液）4.1%，细胞裂解液（总蛋白激酶B重组蛋白，三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4.1%
Components
Eu-Total AKT (25 µL)
Acc-Total AKT (100 µL)
AKT control lysate (200 µL)
5X Lysis Buffer 7 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.
Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 by PDGF-AA but does not affect the levels of total AKT pan.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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