全部商品分类首页
THUNDER™ Total c-Met TR-FRET Cell Signaling Assay Kit
1/2
THUNDER™ Total c-Met TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
用小程序，查商品更便捷
收藏
对比
咨询反应种属：
Human
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
Met
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total c-Met in cell lysates. This kit can be used to normalize data obtained with the Phospho-c-Met (Y1234/Y1235) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-Total MET (25 µL)
Acc-Total MET (100 µL)
MET control lysate (200 µL)
5X Lysis Buffer 5 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human
Data show that treatment of A431 cells with HGF stimulates phosphorylation of c-Met at Y1234/Y1235 but does not affect the levels of total c-Met.
Data show that treatment of HeLa cells with PHA-665752 inhibits phosphorylation of c-Met at Y1234/Y1235 by HGF, but does not affect the levels of total c-Met.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。