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THUNDER™ Total ERK1/2 TR-FRET Cell Signaling Assay Kit
THUNDER™ Total ERK1/2 TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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对比
咨询反应种属：
Human, Mouse
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
ERK1/2
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total ERK1/2 (both phosphorylated and non-phosphorylated ERK1/2) in cell lysates. This kit can be used to normalize data obtained with the Phospho-ERK1/2 (T202/Y204) kit and the Extreme Phospho-ERK1/2 (T202/Y204) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
总细胞外信号调节激酶抗体（铕离子标记），总细胞外信号调节激酶抗体（远红光受体标记），裂解缓冲液 （聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液 （三(羟甲基)氨基甲烷水溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）
Components
Eu-phospho-ERK (25 µL)
Acc-phospho-ERK (100 µL)
ERK control lysate (200 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human, Mouse
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204, but does not affect the levels of total ERK1/2.
Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF, but does not affect the levels of total ERK1/2.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human, Mouse
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