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THUNDER™ Total JNK1/2/3 TR-FRET Cell Signaling Assay Kit
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THUNDER™ Total JNK1/2/3 TR-FRET Cell Signaling Assay Kit

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品牌: Bioauxilium
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反应种属:
Human
产品介绍
产品介绍
产品信息
抗原名称
JNK1/2/3
商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total JNK1/2/3 in cell lysates. This kit can be used to normalize data obtained with the Phospho-JNK1/2/3 (T183/Y185) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Components
Eu-Total JNK (25 µL)
Acc-Total JNK (100 µL)
JNK control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human

Stimulation of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with Anisomycin stimulates phosphorylation of JNK1/2/3 at T183/Y185 but does not affect the levels of total JNK1/2/3.
Inhibition of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with BX 795 inhibits phosphorylation of JNK1/2/3 at T183/Y185 by Anisomycin, but does not affect the levels of total JNK1/2/3.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
应用
反应种属
Human
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货号:
KIT-JNKT-500
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