THUNDER™ Total MEK1 TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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参数

反应种属：

Human

产品介绍

产品信息

抗原名称

MEK1

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total MEK1 in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Components
Eu-Total MEK1 (250 µL)
Acc-Total MEK1 (1 mL)
MEK1 control lysate (2 x 200 µL)
5X Lysis Buffer 3 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)

Reactivity
Human

Stimulation of Phospho-MEK1 (S218-S222) in Hela cells

Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222, but does not affect the levels of total MEK1.

Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells

Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA, but does not affect the levels of total MEK1.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

应用

反应种属

Human

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货号：

KIT-MEK1T-5000

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询价

5000points

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