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THUNDER™ Total NF-kB TR-FRET Cell Signaling Assay Kit
THUNDER™ Total NF-kB TR-FRET Cell Signaling Assay Kit
品牌： Bioauxilium
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咨询反应种属：
Human
产品介绍
产品介绍
产品信息
抗原名称
NF-κB
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total NF-κB (both phosphorylated and non-phosphorylated NF-κB) in cell lysates. This kit can be used to normalize data obtained with the Phospho-NF-κB (S536) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-total-NFkB (250 µL)
Acc-total-NFkB (1 mL)
NFkB control lysate (2 x 200 µL)
5X Lysis Buffer 2 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)
Reactivity
Human
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536, but does not affect the levels of total NF-kB
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα, but does not affect the levels of total NF-kB
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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