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THUNDER™ Total p53 TR-FRET Cell Signaling Assay Kit

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产品介绍
产品介绍
产品信息
抗原名称
p53

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total p53 in cell lysates. This kit can be used to normalize data obtained with the Phospho-p53 (S15) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-Total p53 (5 µL)
Acc-Total p53 (20 µL)
p53 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human

Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at S15, but does not affect the levels of total p53

Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human

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