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THUNDER™ Total p53 TR-FRET Cell Signaling Assay Kit
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THUNDER™ Total p53 TR-FRET Cell Signaling Assay Kit

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品牌: Bioauxilium
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反应种属:
Human
产品介绍
产品介绍
产品信息
抗原名称
p53
商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total p53 in cell lysates. This kit can be used to normalize data obtained with the Phospho-p53 (S15) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Components
Eu-Total p53 (5 µL)
Acc-Total p53 (20 µL)
p53 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at S15, but does not affect the levels of total p53
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human
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货号:
KIT-P53T-100
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