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THUNDER™ Total STAT3 TR-FRET Cell Signaling Assay Kit
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产品介绍
产品信息
抗原名称
STAT3
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total STAT3. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Note: the Total STAT3 Assay Kit uses a new antibody pair that significantly improves the assay performance.
Product citation: PubMed 34570089
Components
Eu-Total STAT3 (250 µL)
Acc-Total STAT3 (1 mL)
STAT3 control lysate (2 x 200 µL)
5X Lysis Buffer 2 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)
Reactivity
Human, Mouse
Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705, but does not affect the levels of total STAT3.
Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα, but does not affect the levels of total STAT3.
Quality Control: the Total STAT3 assay kit is routinely tested against IFNα treated HeLa lysates.
应用
反应种属
Human, Mouse
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