rhTIMP-2, CF (10 ug)

品牌： R&D Systems

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产品介绍

产品信息

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

971-TM

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 50 mM Tris, 10 mM CaCl₂, 150 mM NaCl, and 0.05% Brij-35, pH 7.5.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Recombinant Human TIMP-2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH₂ (Catalog # ES001). The IC₅₀ value is approximately 2.2 nM, as measured under the described conditions.

Source

Chinese Hamster Ovary cell line, CHO-derived human TIMP-2 protein
Cys27-Pro220

Accession #

P16035

N-terminal Sequence
Analysis

Cys27

Predicted Molecular Mass

21 kDa

SDS-PAGE

22-23 kDa, reducing conditions

背景

别名

CSC-21Ktissue inhibitor of metalloproteinase 2,metalloproteinase inhibitor 2,TIMP metallopeptidase inhibitor 2,TIMP2,TIMP-2,Tissue inhibitor of metalloproteinases 2,Tissue Inhibitors of Metalloproteinases 2

背景

Background: TIMP-2

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.

Long Name

Tissue Inhibitors of Metalloproteinases 2

Entrez Gene IDs

7077 (Human); 21858 (Mouse); 29543 (Rat)

Alternate Names

CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2

研究领域

Related Research Areas

制备和贮存

溶解方法

Reconstitute at 100 μg/mL in sterile 50 mM Tris, 10 mM CaCl

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货号：

971-TM-010

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询价

10ug

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