CST_TNF-alpha(D2D4)XP Rabbit mAb(Mouse Specific)_优宁维(univ)在线
现货 TNF-alpha(D2D4)XP Rabbit mAb(Mouse Specific)
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货号: 11948S 品牌: CST 库存: 公司现货
规格: 100ul
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价格: ¥4431.00

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产品信息
来源纯化
使用重组小鼠 TNF-α 蛋白对动物进行免疫接种来产生单克隆抗体。
宿主
Rabbit
应用
敏感性
内源性
背景
背景
TNF 蛋白超家族的典型成员 TNF-α ,是一个 II 型同源三聚体的膜蛋白质 (1,2)。膜结合 TNF-α 被金属蛋白酶 TACE/ADAM17 剪切,可生产一个可溶性同型三聚体 (2)。TNF-α 的膜形态和可溶形态均具有生物活性。TNF-α 由包括 T 细胞、B 细胞、NK 细胞和巨噬细胞在内的许多免疫细胞产生 (1)。针对 TNF-α 的细胞反应通过与受体 TNF-R1 和 TNF-R2 的相互作用介导,并会根据细胞类型和生理背景,激活有利于细胞生存和凋亡的通路。激酶通路(包括 JNK、Erk1/2、p38 MAPK 和 NF-κB)的激活会促进细胞的存活,而 TNF-α 介导的 caspase-8 激活会导致程序性细胞死亡 (1,2)。TNF-α 在炎症和对抗细菌感染的宿主防御中发挥关键的调节作用,尤其是结核分支杆菌 (3)。预计小鼠和大鼠的 TNF-α 可以糖基化,但是人 TNF-α 不会糖基化 (4)。 Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56. Hehlgans, T. and Pfeffer, K. (2005) Immunology 115, 1-20. Lin, P.L. et al. (2007) J Investig Dermatol Symp Proc 12, 22-5. Pennica, D. et al. (1985) Proc Natl Acad Sci U S A 82, 6060-4.
分子量
17,25,28
Entrez-Gene ID
21926
UniProt ID
P06804
抗原
TNF-alpha

参考图片

Confocal immunofluorescent analysis of Raw 264.7 cells, treated with LPS (100 ng/mL, 6 hr; left) or untreated (right), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析Raw264.7细胞,LPS (100 ng/mL, 6 小时; 左图)或未处理(右图),使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (绿色)。肌动蛋白微丝是通过DY-554 phalloidin标记的(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Immunoprecipitation of TNF-α from Raw 264.7 cell extracts, treated with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific). Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as sencondary antibodies.对Raw264.7细胞提取物中的TNF-α进行免疫沉淀,LPS (100 ng/mL, 6小时) 和Brefeldin A #9972 (300 ng/mL, 刺激后3小时)处理,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或 TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (泳道3)。泳道1为10%的加入量。Western blot分析使用的抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific)。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 和Anti-mouse IgG, HRP-linked Antibody #7076都被用来作为二抗。

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated (+) with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析Raw264.7细胞的细胞提取物,未处理(-)或LPS (100 ng/mL, 6 小时) 和Brefeldin A #9972 (300 ng/mL,最后3小时刺激),使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (上图)或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。

Western blot analysis of 1 ng recombinant mouse TNF-α #5178 and 1 ng recombinant rat TNF-α using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific). Western blot分析 1 ng recombinant mouse TNF-α #5178 和 1 ng recombinant rat TNF-α,使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific)。

Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术对Raw264.7进行分析,未处理(蓝色)或LPS (100 ng/ml, 6小时) 和Brefeldin A #9972 (300 ng/ml, 最后3小时处理) (绿色),使用抗体是TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific)。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414用来作为二抗。

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