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TNF-alpha (D2D4) XP ®  Rabbit mAb
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TNF-alpha (D2D4) XP ® Rabbit mAb

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品牌: CST
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分子量:
17,25,28
反应种属:
Mouse
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
TNF-alpha
来源纯化

Monoclonal antibody is produced by immunizing animals with a recombinant mouse TNF-α protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunoprecipitation1:50
Immunofluorescence (Immunocytochemistry)1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)1:100 - 1:400
同种型
Rabbit IgG
分子量
17,25,28
研究领域
癌症,纤维化,免疫学和肿瘤学,神经科学,
应用
反应种属
Mouse
目标/特异性

Specificity/Sensitivity

TNF-α (D2D4) XP Rabbit mAb recognizes endogenous levels of total TNF-α protein.

Species Reactivity:

Mouse

敏感性
Endogenous
背景
背景
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).Mouse and rat TNF-α are predicted to be glycosylated, while human TNF-α is not (4). 1.Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56. 2.Hehlgans, T. and Pfeffer, K. (2005) Immunology 115, 1-20. 3.Lin, P.L. et al. (2007) J Investig Dermatol Symp Proc 12, 22-5. 4.Pennica, D. et al. (1985) Proc Natl Acad Sci U S A 82, 6060-4.
研究领域
癌症,纤维化,免疫学和肿瘤学,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #57485.

数据库链接
Entrez-Gene ID
21926
UniProt ID
P06804

参考图片

Flow cytometric analysis of Raw 264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) and Brefeldin A #9972 (300 ng/ml, last 3 hr of treatment) (green), using TNF-α (D2D4) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated (+) with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using TNF-α (D2D4) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of 1 ng recombinant mouse TNF-α #5178 using TNF-α (D2D4) XP® Rabbit mAb.

Immunoprecipitation of TNF-α from Raw 264.7 cell extracts, treated with LPS (100 ng/mL, 6 hr) and Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or TNF-α (D2D4) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using TNF-α (D2D4) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as sencondary antibodies.

Confocal immunofluorescent analysis of Raw 264.7 cells, treated with LPS (100 ng/mL, 6 hr; left) or untreated (right), using TNF-α (D2D4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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货号:
11948T
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