TNF-α Protein, Mouse

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TNF-α Protein, Mouse

品牌： UA BIOSCIENCE

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参数

分子量：

纯度：

产品介绍

产品信息

荧光素标记

Unconjugated

氨基酸序列

Asp89-Leu235

分子量

17kDa (Reducing)

生物活性

EC50＜15pg/ml

纯度

＞95% by SDS-PAGE and SEC-HPLC

内毒素水平

<0.1EU/μg

性状

Lyophilized Powder

表达系统

E.coli

缓冲体系

20mM PB, 300mM NaCl, 2mM TCEP, pH7.5

标签

No Tag

种属

Mouse

电泳JSON

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2μg (R: reducing condition, N: non-reducing condition).

"}]

背景

别名

NFα蛋白;Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2

背景

Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.

制备和贮存

溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

保存方式

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

文献

1.Microsc Res Tech. 2000 Aug 1;50(3):184-95.

2.Methods Mol Biol. 2021;2248:271-279.

数据库链接

Accession

P06804

参考图片

2μg (R: reducing condition, N: non-reducing condition).

>95% as determined by SEC-HPLC.

Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 15pg/ml.

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货号：

UA040173-100ug

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