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rhTNF-a, CF (2 mg)
rhTNF-a, CF (2 mg)
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rhTNF-a, CF (2 mg)

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品牌： R&D Systems
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    产品介绍
    产品信息

    Scientific Data

    SEC-MALS View Larger

    Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

    Bioactivity Recombinant Human TNF-alpha Protein BioactivityView Larger

    Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.

    SDS-PAGE Recombinant Human TNF-alpha Protein SDS-PAGEView Larger

    1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    210-TA

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    ReconstitutionReconstitute at 0.1-1 mg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    210-TA/CF

    FormulationLyophilized from a 0.2 µm filtered solution in PBS.
    ReconstitutionReconstitute at 0.1-1 mg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Recombinant Human TNF-alpha Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
    Source
    E. coli-derived human TNF-alpha protein
    Val77-Leu233, with and without an N-terminal Met
    Accession #
    P01375
    N-terminal Sequence
    Analysis
    Met & Val77
    Predicted Molecular Mass
    17 kDa
    SDS-PAGE
    17 kDa, reducing conditions
    背景
    背景

    Background: TNF-alpha

    Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

    References
    1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
    2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
    3. Pennica, D. et al. (1984) Nature 312:724.
    4. Tang, P. et al. (1996) Biochemistry 35:8216.
    5. Perez, C. et al. (1990) Cell 63:251.
    6. Black, R.A. et al. (1997) Nature 385:729.
    7. Moss, M.L. et al. (1997) Nature 385:733.
    8. Gearing, A.J.H. et al. (1994) Nature 370:555.
    9. Schall, T.J. et al. (1990) Cell 61:361.
    10. Loetscher, H. et al. (1990) Cell 61:351.
    11. Dembic, Z. et al. (1990) Cytokine 2:231.
    12. Smith, C.A. et al. (1990) Science 248:1019.
    13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
    14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
    15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.
    Long Name
    Tumor Necrosis Factor alpha
    Entrez Gene IDs
    7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
    Alternate Names
    APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

    Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25‑100 pg/mL.

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    货号：
    210-TA-02M/CF
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