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BD OptEIA™ Mouse TNF ELISA Set II
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BD OptEIA™ Mouse TNF ELISA Set II

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品牌: BD Pharmingen
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反应种属:
Mouse (QC Testing)
实验应用:
ELISA (Routinely Tested)
产品介绍
产品介绍
产品信息
抗原名称
TNF
克隆号
(RUO)
BD化合物表
  • 描述
    数量/尺寸
    零件号
    EntrezGene ID
  • Capture Antibody Purified Anti-Mouse TNF
    N/A
    51-9004717
    N/A
  • Detection Antibody Biotin Anti-Mouse TNF
    N/A
    51-9004718
    N/A
  • Recombinant Mouse TNF Lyophilized Standard
    N/A
    51-26986E
    N/A
  • Enzyme Reagent Streptavidin-HRP (SAv-HRP) 250X
    N/A
    51-9004787
    N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
TNF
文献
文献
研发参考(13) 1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology). 2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology). 3. Drew PD, Chavis JA. Female sex steroids: effects upon microglial cell activation. J Neuroimmunol. 2000; 111(1-2):77-85. (Biology). 4. Drew PD, Chavis JA. Inhibition of microglial cell activation by cortisol. Brain Res Bull. 2000; 52(5):391-396. (Biology). 5. Drew PD, Chavis JA.. The cyclopentone prostaglandin 15-deoxy-Delta(12,14) prostaglandin J2 represses nitric oxide, TNF-alpha, and IL-12 production by microglial cells.. J Neuroimmunol. 2001; 115(1-2):28-35. (Biology). 6. Ferran C, Dautry F, Merite S, et al. Anti-tumor necrosis factor modulates anti-CD3-triggered T cell cytokine gene expression in vivo. J Clin Invest. 1994; 93(5):2189-2196. (Biology). 7. He J, Gurunathan S, Iwasaki A, Ash-Shaheed B, Kelsall BL. Primary role for Gi protein signaling in the regulation of interleukin 12 production and the induction of T helper cell type 1 responses . J Exp Med. 2000; 191(9):1605-1610. (Biology). 8. Leiby DA, Fortier AH, Crawford RM, Schreiber RD, Nacy CA. In vivo modulation of the murine immune response to Francisella tularensis LVS by administration of anticytokine antibodies. Infect Immun. 1992; 60(1):84-89. (Biology). 9. Merrick BA, He CY, Craig WA, et al. Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment. Appl Theor Electrophor. 1992; 2(6):177-187. (Biology). 10. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 11. Rabinovici R, Bugelski PJ, Esser KM, et al. Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats. J Pharmacol Exp Ther. 1993; 267(3):1550-1557. (Biology). 12. Sheehan KC, Ruddle NH, Schreiber RD. Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors. J Immunol. 1989; 142(11):3884-3893. (Biology). 13. Takahashi S, Kapas L, Fang J, Krueger JM. An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits. Brain Res. 1995; 690(2):241-244. (Biology).

参考图片

Representative standard curve using the BD OptEIA Mouse TNF ELISA Set II. A standard curve should be ran for each assay.

Typical Standard Curve: The standard curve is for demonstration only. A standard curve must be run with each assay. The standard curve and the 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.

Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (1000 pg/mL), then adding 100 µL of the 1000 pg/mL standard to both that well and the 500 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 500 pg/mL standard to the 250 pg/mL well. Continue these dilutions to the 15.6 pg/mL standard well, out of which the extra 100 µL should be discarded.

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货号:
558534
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